Journal: Protein expression and purification

Article Title: Affimers as an alternative to antibodies for protein biomarker enrichment.

doi: 10.1016/j.pep.2020.105677

Figure Lengend Snippet: Figure 1 (A) graphical overview of alternative splicing of the IL-37 gene that yields different IL-37 protein isoforms and (B) the synthesis of preproinsulin into proinsulin by signal peptidases, which then is cleaved into insulin by prohormone convertase-1 and 2 at the B-chain - C-peptide and A-chain - C-peptide junctions.

Article Snippet: To generate affimer candidates against proinsulin, the library was selected against biotinylated recombinant human proinsulin (R&D Systems), with counter-selection performed against insulin and the C-peptide.

Techniques: Alternative Splicing

Journal: Protein expression and purification

Article Title: Affimers as an alternative to antibodies for protein biomarker enrichment.

doi: 10.1016/j.pep.2020.105677

Figure Lengend Snippet: Figure 4 Graphical illustration of the affimer and antibody-based capture experiments of recombinant proinsulin.

Article Snippet: To generate affimer candidates against proinsulin, the library was selected against biotinylated recombinant human proinsulin (R&D Systems), with counter-selection performed against insulin and the C-peptide.

Techniques: Recombinant

Journal: Developmental Cell

Article Title: Defining the identity and the niches of epithelial stem cells with highly pleiotropic multilineage potency in the human thymus

doi: 10.1016/j.devcel.2023.08.017

Figure Lengend Snippet:

Article Snippet: HumanKine® recombinant human IL-7 protein , Proteintech , cat# HZ-1281.

Techniques: Recombinant, Modification, Selection, RNA Extraction, Polymer, Cell Culture, Derivative Assay, Software